Answer: Unzips the DNA molecule
What is the role of the DNA helicase in the replication of DNA?

DnaB helicase is an enzyme in bacteria which opens the replication fork during DNA replication. Although the mechanism by which DnaB both couples ATP hydrolysis to translocation along DNA and denatures the duplex is unknown a change in the quaternary structure of the protein involving dimerisation of the N-terminal domain has been observed and may occur during the enzymatic cycle. Initially when DnaB binds to dnaA it is associated with dnaC a negative regulator. After DnaC dissociates DnaB binds dnaG.

DNA replication like all biological polymerization processes proceeds in three enzymatically catalyzed and coordinated steps: initiation elongation and termination. For a cell to divide it must first replicate its DNA. DNA replication is an all-or-none process; once replication begins it proceeds to completion. Once replication is complete it does not occur again in the same cell cycle. This is made possible by the division of initiation of the pre-replication complex.

DnaA - Wikipedia

Helicase - Wikipedia

Circular chromosome - Wikipedia

Eukaryotic DNA replication - Wikipedia

Helicase polarity which is also deemed "directionality" is defined as the direction (characterized as 5'→3' or 3'→5') of helicase movement on the DNA /RNA single-strand along which it is moving. This determination of polarity is vital in f.ex. determining whether the tested helicase attaches to the DNA leading strand or the DNA …

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