What types of media of commonly used to isolate anaerobic microbes?
The most common growth media for microorganisms are nutrient broths (liquid nutrient medium) or lysogeny broth medium. Liquid media are often mixed with agar and poured via a sterile media dispenser into Petri dishes to solidify. These agar plates provide a solid medium on which microbes may be cultured. They remain solid as very few bacteria are able to decompose agar (the exception being ...
Anaerobes have been found in infections throughout the human body. The frequency of the host or patient's recovery depends on the employment of proper methods of collection of specimen their transportation to the microbiology laboratory and cultivation. The recovery of organisms depends on the site of infection and is related to the adjacent mucous membranes microbial flora. Anaerobes are able to cause all types of intracranial infections. These often cause subdural empyema …
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Some bacteria like Legionella species require particular nutrients or toxin binding as in charcoal to grow and therefore media such as Buffered charcoal yeast extract agar must be used. If one wants to isolate as many or all strains possible different nutrient media as well as enriched media such as blood agar and chocolate agar and anaerobic culture media such as thioglycolate broth need to be inoculated.
Sodium polyanethol sulfonate (SPS) is the most commonly used anticoagulant because it does not interfere with the growth of most organisms. The exact composition of the growth medium varies but aerobic bottles use a broth that is enriched with nutrients such as brain-heart infusion or trypticase soy broth [34] and anaerobic bottles typically contain a reducing agent such as thioglycollate .
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