Answer: T
Flow cytometry makes use of fluorescence to count microbes. T or F?

Flow cytometry (FC) is a technique used to detect and measure physical and chemical characteristics of a population of cells or particles. In this process a sample containing cells or particles is suspended in a fluid and injected into the flow cytometer instrument. The sample is focused to ideally flow one cell at a time through a laser beam where the light scattered is characteristic to the cells and their com…

Flow cytometry (FC) is a technique used to detect and measure physical and chemical characteristics of a population of cells or particles. In this process a sample containing cells or particles is suspended in a fluid and injected into the flow cytometer instrument. The sample is focused to ideally flow one cell at a time through a laser beam where the light scattered is characteristic to the cells and their components. Cells are often labeled with fluorescent markers so light is absorbed and then emitted in a band of wavelengths. Tens of thousands of cells can be quickly examined and the data gathered are processed by a computer. Flow cytometry is routinely used in basic research clinical practice and clinical trials. Uses for flow cytometry include: Cell countingCell sortingDetermining cell characteristics and functionDetecting microorganismsBiomarker detectionProtein engineering detectionDiagnosis of health disorders such as blood cancersA flow cytometry analyzer is an instrument that provides quantifiable data from a sample. Other instruments using flow cytometry include cell sorters which physically separate and thereby purify cells of interest based on their optical properties.

The first impedance -based flow cytometry device using the Coulter principle was disclosed in U.S. Patent 2 656 508 issued in 1953 to Wallace H. Coulter . Mack Fulwyler was the inventor of the forerunner to today's flow cytometers - particularly the cell sorter. Fulwyler developed this in 1965 with his publication in Science . The first fluorescence-based flow cytometry device (ICP 11) was developed …

The first impedance -based flow cytometry device using the Coulter principle was disclosed in U.S. Patent 2 656 508 issued in 1953 to Wallace H. Coulter . Mack Fulwyler was the inventor of the forerunner to today's flow cytometers - particularly the cell sorter. Fulwyler developed this in 1965 with his publication in Science . The first fluorescence-based flow cytometry device (ICP 11) was developed in 1968 by Wolfgang Göhde from the University of Münster filed for patent on 18 December 1968 and first commercialized in 1968/69 by German developer and manufacturer Partec through Phywe AG in Göttingen . At that time absorption methods were still widely favored by other scientists over fluorescence methods. Soon after flow cytometry instruments were developed including the Cytofluorograph (1971) from Bio/Physics Systems Inc. (later: Ortho Diagnostics) the PAS 8000 (1973) from Partec the first FACS (fluorescence-activated cell sorting) instrument from Becton Dickinson (1974) the ICP 22 (1975)...

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